von Willebrand Factor Gene Polymorphism in Preeclampsia Pregnant at Medan, Indonesia

BACKGROUND: von Willebrand Factor (vWF) is a large glycoprotein mediating hemostasis and thrombosis. The roles of vWF are platelets adhesion to sites vascular damage stabilization coagulation factor VIII. AIM: This study aimed analyze the polymorphism gene on preeclampsia (PE) in pregnancy Medan, Indonesia. MATERIALS AND METHODS: DNA was amplified using polymerase chain reaction electrophoresed agarose 2%. Electrophoresis results were detected Gel Doc 1000 (Biorad, USA). sequencing method used identify from gene. RESULTS: From 50 samples PE patients, g.93308C>T found with percentage TT, CT, CC genotypes as 50%, 42%, 8%, respectively. CONCLUSION: c.93308C>T genotype homozygous heterozygote CT greater than wild-type CC.